Research Highlights

Liu, L., Kitova, E.N., and Klassen, J.S. Quantifying Protein-Fatty Acid Interactions using Electrospray Ionization Mass Spectrometry. 
J American Society for Mass Spectrometry 22, 310-318, 2011.

El-Hawiet, A., Kitova, E.N., Liu, L., and Klassen J.S. Quantifying Labile Protein-Ligand Interactions using Electrospray Ionization Mass Spectrometry. Journal of the American Society for Mass Spectrometry 21, 1893-1899, 2010.

Deng L, Sun N, Kitova EN, Klassen JS. Direct quantification of protein-metal ion affinities by electrospray ionization mass spectrometry.  Anal. Chem. 82: 2170-2174, 2010.  

Soya, N., G.K. Shoemaker, M.M. Palcic, J.S. Klassen. Comparative Study of Substrate and Product Binding to the Human ABO(H) Blood Group Glycosyltransferases. Glycobiology 19, 1224-1234, 2009.

Bagal, D., E.N. Kitova, L. Liu, A. El-Hawiet, P.D. Schnier, J.S. Klassen. Gas phase sStabilization of noncovalent protein complexes formed by electrospray ionization. Anal Chem, 81, 7801-7806, 2009.

Kitova, E., G.L. Mulvey, T. Dingle, S. Wee, T.P. Griener, G.L. Armstrong, J.S. Klassen. Assembly and stability of the Shiga toxins investigated by temperature-controlled electrospray ionization mass spectrometry. Biochemistry48, 5365-5374, 2009.

Humphries, R.M., M.S. Donnenberg, J. Strecker, E. Kitova, J.S. Klassen, L. Cui, T.P. Griener, G.L. Mulvey, G.D. Armstrong. From alpha to beta: identification of amino acids required for the N-acetyllactosamine-specific lectin-like activity of bundlin. Mol Microbiol 72, 859-864, 2009.

Shoemaker, G.K., N .Soya, M.M. Palcic, J.S .Klassen. Temperature dependent cooperativity in donor-acceptor substrate binding to the human blood group glycosyltransferases. Glycobiology 18, 587–592, 2008.

Dingle, T., S. Wee, G. L. Mulvey, A. Greco, K. Ng, J S. Klassen, G. D. Armstrong. Functional properties of the carboxy-terminal host cell binding domains of the two toxins, TcdA and TcdB, expressed by Clostridium difficile.Glycobiology 18, 698–706, 2008.

Kitov, P. I., T. Lipinski, E. Paszkiewicz, D. Solomon, J.M. Sadowska, G.G.A. Grant, G.L. Mulvey, E.N. Kitova, J.S. Klassen, G.D. Armstrong, D R. Bundle. An entropically efficient supramolecular inhibition strategy for shiga toxins.Angew Chemie  Intl Ed 47, 672–676, 2008.

Hyland, R.M., J. Sun, T.P. Griener, G.L. Mulvey, J.S. Klassen, M. Donnenberg and G.D. Armstrong.  Bundlin pilin protein of enteropathogenic Escherichia coli is an N-acetyllactosamine Specific Lectin. Cell Microbiol 10, 177–187, 2008.

Faridmoayer, A., M.A. Fentabil, D.C. Mills, J.S. Klassen, M.F. Feldman. Functional characterization of bacterial oligosaccharyltransferases involved in O-linked protein glycosylation. J Bacteriol 189, 8088-8098, 2007.

Sun, J., Kitova, E.N., Sun, N.; and  Klassen, J.S. Method for Identifying Nonspecific Protein-Protein Interactions in Nanoelectrospray Ionization Mass Spectrometry. Anal. Chem. 79, 8301-8311, 2007.

Kitova, E.N., P.I. Kitov, E. Paszkiewicz, J. Kim, G.L. Mulvey, G.D. Armstrong, D.R. Bundle, J.S. Klassen. Affinities of shiga toxins 1 and 2 for univalent and oligovalent Pk trisaccharide analogs measured by electrospray ionization mass spectrometry. Glycobiology , 17: 1127–1137, 2007.

Rademacher, C., G.K. Shoemaker, H-S. Kim, R.B. Zheng, C. Liu, R. Nacario, J.S. Klassen, T. Peters, T.L. Lowary. Ligand specificity of cs-35, a monoclonal antibody that recognizes mycobacterial lipoarabinomannan. A model system for oligofuranoside-protein recognition. J Am Chem Soc 129:10489-10502, 2007.

Sun, J., Kitova, E.N., Sun, N.; and  Klassen, J.S.  Method for identifying nonspecific protein-protein complexes in nanoelectrospray ionization mass spectrometry. Analytical Chem 79:416-425, 2007.

Sun, J., Kitova, E.N., Wang, W.; and  Klassen, J.S. Method for Distinguishing Specific and Nonspecific Protein-Ligand Complexes in Nanoelectrospray Ionization Mass Spectrometry. Anal. Chem. 78, 3010-3018, 2006.

Daneshfar, R., Kitova, E.N., and  Klassen, J.S. Determination of Protein-Ligand Association Thermochemistry Using Variable-Temperature Nanoelectrospray Mass Spectrometry. J. Am. Chem. Soc. 126, 4786-4787, 2004.

Wang, W., Kitova, E.N., and  Klassen, J.S. Influence of Solution and Gas Phase Processes on Protein-Carbohydrate Binding Affinities Determined by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry. Anal. Chem. 75, 4945-4955, 2003.